Kirksville Regional Airport (IATA: IRK, ICAO: KIRK, FAA LID: IRK) is four miles south of Kirksville, Missouri, on the west side of US highway 63. One airline schedules passenger flights, subsidized by the Essential Air Service program.

Federal Aviation Administration records say the airport had 684 passenger boardings (enplanements) in calendar year 2008, 926 in 2009 and 2,127 in 2010. The National Plan of Integrated Airport Systems for 2011–2015 categorized it as a general aviation facility (the commercial service category requires at least 2,500 enplanements per year).

Cape Air is the current airline, averaging 900 to 1,000 passengers per month on three daily round trips to St. Louis Lambert International Airport. After Cape Air notified the city in November 2022 of their plans to terminate their service, the Kirksville City Council approved a contract on February 6, 2023 with Contour Airlines, with service beginning in June to Chicago O'Hare International Airport.